There is a nice paper describing various systems that have been designed to insure against the "escape" of genetically modified organisms. The Hok system is mentioned along with data on the number of microbes that "escape" the selection. The number of escapees seems pretty high for Hok-if your goal is insuring against escape you might want to look at some of the other systems described. Here's a link to the paper: http://www.frontiersin.org/Microbiotechnology,_Ecotoxicology_and_Bioremediation/10.3389/fmicb.2013.00005/abstract
On Sunday, March 24, 2013 2:10:21 PM UTC-4, Koeng wrote:
Hello everyone! I was recently planning out a synthesis to do, and I wanted to get the best bang for my buck. I am going to use gene art strings, and I am going to get 450$ worth, 3,000 bp, but my gene is only 2338 bps long. So, I wanted some ideas to fill up the rest of the space. Right now I am thinking of putting the Hok/Sok toxin antitoxin system... but thats pretty much it. Any ideas for mini-genes that would be all around useful, or interesting? Please just post them below!---Koeng
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