I could fuse a signal peptide to the end of hok... but then again the antitoxin still destroys hok.
Also I will look into synthesizing relBE!
I never really... hehe... try to ensure that my bacteria wouldn't escape, I mean where is K12 going to go if not onto my petri dish!
-Koeng
On Monday, March 25, 2013 3:09:03 AM UTC-7, Mega wrote:
-- On Monday, March 25, 2013 3:09:03 AM UTC-7, Mega wrote:
What if you add a signal peptide so it gets secreted???Can the other bacteria still take it up as the entire protein? (Of course the signal peptide has to be cleaved off, else it would carry the signal again to be secreted!!)
On Sunday, March 24, 2013 7:10:21 PM UTC+1, Koeng wrote:Hello everyone! I was recently planning out a synthesis to do, and I wanted to get the best bang for my buck. I am going to use gene art strings, and I am going to get 450$ worth, 3,000 bp, but my gene is only 2338 bps long. So, I wanted some ideas to fill up the rest of the space. Right now I am thinking of putting the Hok/Sok toxin antitoxin system... but thats pretty much it. Any ideas for mini-genes that would be all around useful, or interesting? Please just post them below!-Koeng
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