Hi Caleb,
We have pGreenII plasmid (binary system) here... The JIC (from UK) sells it for I think 40 or 60 british pounds or so.
At the moment we are trying to insert the 35S-GFP into it, but it just doesn't wanna work. Blunt end ligation is awful, additionally some of the enzymes in our lab are so old you have to add 30* the usual ammount...
That would give pGreenII-0049-Luc-35S-GFP construct...
Well, when the Genome Compiler kickstarter campaign runs off, we won't need GFP any more though...
Best,
Andreas
Am Freitag, 8. März 2013 01:26:49 UTC+1 schrieb Caleb:
As the title says, this I was I'm looking to do, but one of the main issues I'm having is finding a source that sells the right plasmids for a decent cost. The main gene of interest here is the Green fluorescent protein (GFP, or red, blue, violet fluorescent). I looked on the addgene website, since I had read that that may be a good source, but I wasn't sure if any of the plasmids that they listed had the t DNA function for the Agrobacterium to work. I also looked on the pGreen website, which has plasmids designed for Agrobacterium, but i wasn't able to find a pricepoint on the website, and I wasn't able to contact them at the time. Getting the bacteria shouldn't be a problem, but I am still considering what plant I will use for the experiment. I'm still doing heavy research on the topic, and any feedback would be tremendously appreciated, also let me know if anything needs to be explained better.--
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