Re: [DIYbio] 6 minute electrophoresis without buffer

On Thu, Mar 7, 2013 at 12:02 PM, Avery louie <inactive.e@gmail.com> wrote:
> Could you just cast your electrodes into your gel? Will anything bad
> happen? I guess it would be hard to remove the electrodes, since they are
> fairly delicate.
>

the patent say gas evolution at the electrode will cause increased
resistance and eventually breaking the circuit unless aluminum and
palladium electrodes are used at opposite ends to absorb the O2 and
H2 gas.

> --A
>
> On Thu, Mar 7, 2013 at 2:07 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>>
>> Seems like it's just a marketing tactic, the second instruction says:
>> "Remove combs, add 300ml deionized water, add little running buffer at
>> both ends, or without running buffer when runing bufferless genotyping
>> gel."
>>
>> That most likely means the gel is buffered too. I think more
>> appropriate marketing would be 'liquid-free', if anything.
>>
>> Here's a patent that's about to expire on this type of system
>> (probably the same as the Invitrogen eGel)
>> http://www.google.com/patents?hl=en&lr=&vid=USPAT5209831
>>
>>
>> I'm trying to figure out what kind of buffer they're using, that's the
>> key to this '6 minute run'... but it's probably Sodium Borate or
>> Lithium Borate (i.e. Faster Better Media LLC) here's 'their' patent:
>> http://www.6mgel.com/6mgel%20patent.pdf
>>
>> And the work Faster Better Media LLC based their products on:
>> http://en.wikipedia.org/wiki/SB_buffer
>>
>> http://www.biotechniques.com/multimedia/archive/00037/BTN_A_04374ST04_O_37093a.pdf
>>
>>
>> --
>> -Nathan
>>
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>
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--
-Nathan

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