Re: [DIYbio] Creating synthetic organelles

Found this, looks open-access:

Internalization of bioluminescent Escherichia coli and Salmonella
Montevideo in growing bean sprouts

K. Warriner, S. Spaniolas, M. Dickinson, C. Wrightand W.M. Waites
Divisions of Food Sciences, Plant Science, and Agriculture and
Horticulture, School of Biosciences, University of Nottingham,
Leicestershire, UK
2003/0154: received 25 February 2003, revised 23 April 2003 and
accepted 4 May 2003
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2003.02037.x/full


On Tue, Mar 5, 2013 at 10:59 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Mon, Mar 4, 2013 at 5:19 PM, Koeng <koeng101@gmail.com> wrote:
>> A few days ago I had an idea when I was searching about chloroplasts (and
>> how people use the PEG i have to put them into plants :). Here it is-
>>
>> Would it be possible to insert modified E coli that exported the protein for
>> resistance to chloramphenicol, therefore making the E coli like a big BAC?
>
> I think the problem with that approach is that the yeast would still
> be relatively unconnected to the e.coli, or connected in the wrong
> way. As Mega pointed out, the plant might see the e.coli inside and
> start attacking, or simple apoptose itself. The e.coli would simply be
> e.coli inside a yeast cell, so I think there would be a lot more work
> to get it hooked up as a sub-yeast worker.
>
> Another problem might be that the photosynthetic leaf cells probably
> would grow more slowly since the photosystems are being blocked
> partially by non-photosynthetic e.coli... so maybe you could find a
> way to only add the e.coli worker to non-photosynthetic cells?
>
>
> Why not start with the plastid instead, and look at modifying a
> version of it. It would be really cool to have a custom organelle to
> do chemistry in, away from the other cell contents!
>
>
>
> --
> -Nathan



--
-Nathan

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