Re: [DIYbio] Mini genes

On Sun, Mar 24, 2013 at 2:35 PM, Jeswin <phillyj101@gmail.com> wrote:

>> am going to get 450$ worth, 3,000 bp, but my gene is only 2338 bps long. So,
>> I wanted some ideas to fill up the rest of the space. Right now I am
>> thinking of putting the Hok/Sok toxin antitoxin system... but thats pretty
>
> Tell me about this Hok/Sok system. I looked at the wikipedia to get an
> idea of what it is. What purpose would you use it for? Just curious.

Did some more reading on it. So it helps you do more efficient cell
culturing? Its a kill-mechanism, isn't it. It ensures that only
bacteria with the plasmid survive. So there are less worries about
running out of antibiotics in culture?

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