Re: [DIYbio] Mini genes

No, I am not creating a new plasmid ( I think it is better to just pcr plasmids and Gibson them together to make minimal plasmids)
The hok/sok system is a plasmids selfish gene, once you get the plasmid YOU NEVER get RID of it >:)

Pretty much I am synthesizing it cause of the old topic on creating a plasmid with antibiotic synthesis and resistance genes, making it so you don't need antibiotics in media. Idk how it would work, so any other genes I would be interested in

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