I was impatient and ran it at like 160V and 60-70? mA and the first four lanes (neg control, opentaq, opentaq, ladder) were loaded first and had a lot more time to settle and diffuse. As you can see, the ladder also streaked, so we are not sure what it is, but we think it has something to do with either the voltage/current settings and a lot of DNA, or the loading.
I want to stress that this is not meant to be a comparison between the two enzymes, as they were run under completely different conditions- just thought people might be curious.
--A
On Mon, Mar 4, 2013 at 11:43 AM, Jeswin <phillyj101@gmail.com> wrote:
On Mon, Mar 4, 2013 at 11:28 AM, Avery louie <inactive.e@gmail.com> wrote:What's with the streaking?
>
> The NEB and Opentaq samples were run on different machines, but this gel
> shows good amplification using the opentaq master mix, even after it was
> left out at room temp for a day+
>
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