I have access to a small soxhlet extractor. If I could clean up agar, I'd sell it to people here for enough to cover the basic costs. Where is that protocol? What's the reality of the cost in time, reagent loss cost (ethanol evaporating, etc), and electricity?
On Mar 23, 2013 9:22 AM, "Cathal Garvey (Phone)" <cathalgarvey@cathalgarvey.me> wrote:
-- --Oh purification for micro is pretty pointless, I was talking about agarose purification for gels.jarlemag <jarle.pahr@gmail.com> wrote:According to a recent article (http://www.ncbi.nlm.nih.gov/pubmed/22809873), food-grade agar can be substituted for bacteriological agar with no apparent effects except colour of the medium.
-J
kl. 10:01:51 UTC+1 lørdag 23. mars 2013 skrev Cathal Garvey (Phone) følgende:I really should add that to my biohacking protocols repo..
Suffice to say it's doable, and straightforward, but very messy and not cost effective unless you can fractionally distil an alcohol *and* propylene glycol (boils 200+C).
Am pretty sure I shared the protocol on this list previously..Patrik D'haeseleer <pat...@gmail.com> wrote:On Monday, March 23, 2009 10:35:28 AM UTC-7, Aaron Hicks wrote:
Some of the older texts (1960s and 1970s) discuss purification of agar to increase clarity. It's a pretty involved process, but if anybody would like the references so they can go read about it themselves, I can look them up.
If I remember correctly, Cathal gave that a try. Don't remember if he ever gave a thorough summary of his experience with this, other than that it was very messy...
Patrik
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