Re: [DIYbio] Re: Does anyone have a / access to a proofreading polymersase?

NEB has dished out free samples to me too, when I get $$$ for some
serious research I'll definitely buy from them. I also recommend them
to lots of folks because of this reason, so if you're at a big
University or company, it might be cheap advertising for them.

On Fri, Mar 22, 2013 at 8:40 AM, poli <polinere@gmail.com> wrote:
> Call NEB and ask for a free sample of Q5
> https://www.neb.com/products/polymerases-and-amplification-technologies/pcr-polymerases/q5-high-fidelity-dna-polymerases/detail.
> My lab has gotten about 5 free samples though we can get phusion for $127
> per 200 rxn.
>
>
> On Tuesday, March 19, 2013 2:07:40 PM UTC-4, Mega wrote:
>>
>> Hi everyone,
>>
>> I soon need to do a PCR of a ~10 kbp fragment.
>>
>> I asked in every lab of my university if they had some proofreading
>> polymerase (like Pfu). Because the error rate of Taq polymerase is somewhat
>> like 1:9000, it won't work with > 3 kbp.
>>
>> Buying one myself may not be an option, because I was told that those are
>> remarkably expensive... (Althought, here it seems quite affordable...
>> http://www.thermoscientificbio.com/pcr-enzymes-master-mixes-and-reagents/pfu-dna-polymerase/
>> 60€ for 100 units )
>>
>>
>> Of course, I'd pay the mailing fee, and a little compensation. Or tade it
>> for one of my plasmids. pVIB, pGreenII-0049, p35S-GFP. Have acces to
>> pTracer-CMV2, so I could possibly share also this one.
>>
>>
>> Just shoot me a mail if interested ;)
>
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--
-Nathan

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