It would probably be better to find a company closer to your country. I've always received samples completely free of any charge. Asking to pay shipping would help your case if they don't wanna ship to you. I've always received cold stuff in Styrofoam boxes packed with dry ice.
On Mar 22, 2013 1:25 PM, "Andreas Sturm" <masterstorm123@gmail.com> wrote:
-- You think they would send it to my university for free?? Even shipping included?
Another company in Europe told me that shipping polymerase has to be on wet ice, costing additional 19€.On Fri, Mar 22, 2013 at 7:06 PM, Nathan McCorkle <nmz787@gmail.com> wrote:NEB has dished out free samples to me too, when I get $$$ for some
serious research I'll definitely buy from them. I also recommend them
to lots of folks because of this reason, so if you're at a big
University or company, it might be cheap advertising for them.
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On Fri, Mar 22, 2013 at 8:40 AM, poli <polinere@gmail.com> wrote:
> Call NEB and ask for a free sample of Q5
> https://www.neb.com/products/polymerases-and-amplification-technologies/pcr-polymerases/q5-high-fidelity-dna-polymerases/detail.
> My lab has gotten about 5 free samples though we can get phusion for $127
> per 200 rxn.
>
>
> On Tuesday, March 19, 2013 2:07:40 PM UTC-4, Mega wrote:
>>
>> Hi everyone,
>>
>> I soon need to do a PCR of a ~10 kbp fragment.
>>
>> I asked in every lab of my university if they had some proofreading
>> polymerase (like Pfu). Because the error rate of Taq polymerase is somewhat
>> like 1:9000, it won't work with > 3 kbp.
>>
>> Buying one myself may not be an option, because I was told that those are
>> remarkably expensive... (Althought, here it seems quite affordable...
>> http://www.thermoscientificbio.com/pcr-enzymes-master-mixes-and-reagents/pfu-dna-polymerase/
>> 60€ for 100 units )
>>
>>
>> Of course, I'd pay the mailing fee, and a little compensation. Or tade it
>> for one of my plasmids. pVIB, pGreenII-0049, p35S-GFP. Have acces to
>> pTracer-CMV2, so I could possibly share also this one.
>>
>>
>> Just shoot me a mail if interested ;)
>
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-Nathan
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