Re: [DIYbio] Re: Genome editing tools, gene deletion?

Splicer complexes like miRNA antisense systems are fairly exclusive to RNA, I think, although you could probably (if so, someone may have already) hack it otherwise.

To selectively excise a chunk of DNA while closing the ends neatly, you could use a integrase/excisionase system, but for the the flanking sequences and resulting 'scar' site must be predefined according to the enzyme.

There's a class of restriction enzyme that excises a chunk of target DNA,sometimes without requiring or leaving boundaries, but they don't repair the free ends. Fusing one to a ligase might help by ensuring that DNA only gets excised when a repair enzyme is close at hand.