Re: [DIYbio] Re: would anyone be interested in cheap PDA?

On Tue, Mar 19, 2013 at 3:05 PM, Dakota Hamill <dkotes@gmail.com> wrote:
> The best part about making PDA at home is getting to eat mashed potatoes
> each time you do it.
>
> I got a gallon of potato water 2 weeks ago after I made some mashed potatoes
> and it's still in a container in my basement, I'll check to see if anything
> is growing in it when I get home. The liquid you get from draining the
> potatoes is a cloudy suspension, and after a day a good 2 inches of "potato
> stuff" settled on the bottom.

I've always used the filtrate directly for broth and agar

>
> The liquid broth seems easy enough to make, but, what part do you dehydrate
> if you wanted to keep it dry and store it? The potato filtrate? The potato
> precipitate?

I imagine everything, but the top layer would probably be fine too.

> Or could you mash up the potatoes and spread them out on a
> cookie sheet in a warm oven and dry them that way, then coffee grinder them
> into a fine powder, add the agar and dextrose accordingly, and BAM. POTATO
> MEDIA HOT OFF THE PRESS.
>
> Or get dehydrated mash potatoes from the grocery store for a dollar, and add
> agar and dextrose accordingly, and then sell 250g and 500g containers for
> comparable online prices.
>
> THE BIRTH OF A POTATO EMPIRE.
>
> How could you sterilize it though? Pressure sterilizer seems like it would
> just make it wet again. Maybe a hot oven for a long period would work.

Why is wetting it a problem? Most/all media has water in it, being the
solvent of life and all.

--
-Nathan

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