Re: [DIYbio] trouble loading onto agarose gel

I did a restriction enzyme cut of a pcr product and wanted to run the
gel and purify it. After adding the loading dye, I proceeded to put
the mix into the well. Unlike other times that I loaded, the mix just
seemed to float away. Only by being extremely careful and extremely
slow was I able to load a good amount. Usually I load 25uL in the well
but this time, I had to load all 45 uL and I think most of it didn't
get in the well.

The last time this happened, I was told it was ethanol contamination
from purification so I took utmost care to reduce this. I was doing
double digests so I did PCR clean up after the first cut. The only
thing different with this particular cutting is that I used Roche NheI
and SuRE cut Buffer M. The NheI had an odor to it; an organic smell
like DMSO. I have not had problems with NEB RE.

Any ideas?

Thanks

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