Thanks...
The promoter should be attached this way. Another PCR for the primer makes it more complicated...
-- On Wed, Mar 6, 2013 at 9:01 AM, Cory Tobin <cory.tobin@gmail.com> wrote:
I get my primers from either IDT or Valuegene. Costs around 0.35USD per base.
Although I doubt you will find anyone who can make a 200base primer.
IDT and Valuegene max out at 100.
Why do you want to make a 200base primer? You usually only need like
25-30 bases for PCR.
-cory
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