Most competent cells are just cells in a solution of counter ions to help alleviate the the charge on DNA passing through a membrane.
I would assume their transformation efficiency is very very low. And the amount of time and effort saved would be expended twofold by needing to incubate the cells with plasmid for such a long period of time and colony PCRing/sequencing many colonies to find a hit.
On Tuesday, April 2, 2013 11:01:38 AM UTC-5, Mega wrote:
I can ask him, when I see him next time.--Next lecture would be tomorrow, however, I am at the AEC to transform agrobacterium with an empty pGreen vector (that carries KanR and firefly luciferase).So, maybe within the next couple of days...
On Tuesday, April 2, 2013 3:17:41 PM UTC+2, Mega wrote:Good news, everyone.A proffessor of mine told me that some microbiologists were laughing at them because they used competent e.coli.
Those microbiologists just incubated the colis with the plasmid for an half hour and then selected. There always were some colonies...
of course, this won't work when doing ligation, but for amplyfying a plasmid it is said to do its job...
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