Ampicillin induction? Is there any operators for that?
Also with the organisms that are minimal I believe are resistant to ampicillin :(
-Koeng
On Tuesday, April 9, 2013 12:05:14 AM UTC-7, Mega wrote:
-- On Tuesday, April 9, 2013 12:05:14 AM UTC-7, Mega wrote:
Brought me of a nice idea: A talen-coding gene, induced by tetracycline. The Talen destroys the Ampicillin gene. Thus you could insert a cassette with a amp marker, select on amp, put the transformants onto tet-agar, and do another integration of a casette.
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