I don't think there is a bacterial strain called DM109. Perhaps are you referring to JM109? Further, JM109 is used specifically for transformation and plasmid DNA isolation and has many genetic manipulations for such.
Also, I would say if you are expressing GFP most of these plasmids require a T7 polymerase which "normal" E. coli dooesn't have.
I am sure as you have said these things can work. But why would anyone even try when it takes just as much effort to do it the "correct" way and perhaps even less effort in the long run due to lack of needing to retransform because poor transformation efficiency.
Take some cells add some 5% PEG and a little Mg or Ca and voila your transformation efficiency increases by many orders of magnitude. I would dissuade people from doing as you said unless they are stranded in a Jungle with no access to any mono or divalent cations.
On Thu, Apr 4, 2013 at 8:52 AM, Mega <masterstorm123@gmail.com> wrote:
Yeah, for ligation it won't be worth surely. but for demonstrating gfp, why not...
I asked him, he said those were just some routine colis. DM109 or something thelike.
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