On Sunday, May 19, 2013 11:53:19 PM UTC-7, Mega wrote:
Tito, There are 5 levels of organisms you coud work with.S0 means it's unregulated. Like looking into a frog, dissecring a cow eye, breeding (potential mycotoxin containing) mould and fungi.
S1 is for GMOs that don't pose a risk.
S2 is for gmos / organisms which may cause a weak risk.
S3 for organisms which cause a risk (Ebola) but the mechsnism of infection is known, so you can protect yourself properly
S4 for unknown pathogens (you get to wear a space suit within S4 labs)
I think we all agree all above S1 isn't DIY Bio anymore
No, we would not all agree on that! In fact, I know of at least two DIYbio groups that are hoping to set up a BSL-2 lab. For one, you're not supposed to generate environmental isolates (e.g. cellulose degrading bacteria from compost) in BSL-1.
There are also some mildly pathogenic (and easily treatable) organisms that are very interesting from a biotechnology perspective. For example, Chromobacterium violaceum makes a beautiful dark blue pigment, and was used in the 2009 "E. chromi" Cambridge IGEM project. But it is also a BSL-2 organism. Interestingly, it is actually available in some sets of pigmented bacteria from educational suppliers such as eNasco and Flinn Scientific.
I would definitely recommend that people do not use BSL-2 organisms in any projects in their kitchen lab, but there's no reason that a well-organized DIY lab, with the right equipment and training, couldn't do BSL-2 work.
In terms of safety, it's probably no worse than working with concentrated sufuric acid or NaOH in a DIY chemistry lab.
Patrik
-- There are also some mildly pathogenic (and easily treatable) organisms that are very interesting from a biotechnology perspective. For example, Chromobacterium violaceum makes a beautiful dark blue pigment, and was used in the 2009 "E. chromi" Cambridge IGEM project. But it is also a BSL-2 organism. Interestingly, it is actually available in some sets of pigmented bacteria from educational suppliers such as eNasco and Flinn Scientific.
I would definitely recommend that people do not use BSL-2 organisms in any projects in their kitchen lab, but there's no reason that a well-organized DIY lab, with the right equipment and training, couldn't do BSL-2 work.
In terms of safety, it's probably no worse than working with concentrated sufuric acid or NaOH in a DIY chemistry lab.
Patrik
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