Re: [DIYbio] Challenges building a DNA synthesizers?

Some of the really cool ideas in research are using nanochannels to
isolate diffusion to as little of the growing end of the DNA chain,
but otherwise the wikipedia article on Oligonucleotide synthesis
covers what J Adams said plus a lot of the history.
http://en.wikipedia.org/wiki/Oligonucleotide_synthesis

On Tue, May 21, 2013 at 2:37 PM, J Adams <jadams@azcobiotech.com> wrote:
> A synthesizer by it's very nature is an automated fluid delivery devise that
> delivers fluids in a specific sequence with specific timing to provide
> automated DNA and RNA synthesis. The process that the machines ALL run are
> Deprotection (i.e. remove the 5' Trityl group), coupling (extend 1 base,
> with activator), capping (an acetylation reaction requiring acetic anhydride
> and NMI) and oxidation (convert the P3 to a stable P5), then they repeat
> this cycle over and over again for the full length of the oligonucleotide
> being synthesized.
>
>
>
> So, ALL SYNTHESIZERS ARE THE SAME. There are slight differences, but, all
> are fluid delivery modules that deliver fluid in an automated fashion to
> perform automated DNA and RNA synthesis.
>
>
>
> I hope this helps.
>
>
>
> Regards;
>
>
>
>
>
>
>
>
>
> J Adams
>
>
>
> From: diybio@googlegroups.com [mailto:diybio@googlegroups.com] On Behalf Of
> Open BioLab Graz - Austria
> Sent: Tuesday, May 21, 2013 2:17 PM
> To: diybio@googlegroups.com
> Subject: [DIYbio] Challenges building a DNA synthesizers?
>
>
>
> Hiho,
>
>
>
>
>
> Does anyone here maybe know what the challenges in building a dna
> synthesizer are?
>
> I havent really got insight into this specific technique, but it seems as if
> these machines just pump a series of liquids..
>
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--
-Nathan

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