Re: [DIYbio] Re: qPCR fluorescence detection dynamic range

>
> But there are applications well beyond that. Health apps are obvious: qPCR
> is the gold standard in viral diagnostics and quantifying viral load. But

Yep, that seems to be the most common uses for qPCR. Viral detection,
mutation assays. The company I work for, Reniguard, has been working
on these kind of assays.

> also say you want to know if there is horse meat in your food, or dolphin
> meat, or listeria, or E.Coli O157:H7? Again qPCR is the gold standard.
>

I thought about that sort of stuff, on the DIYbio level. But what
makes qPCR a better alternative than regular PCR and running a gel?
Getting cheap qPCR enzymes that also give good signals are difficult.
You can't use regular Taq.

> But I'm even interested in the most mundane uses. Say you have ran a PCR
> reaction, and now want to send it off for sequencing to do DNA barcoding. Or
> you PCRed something to clone. But you don't know if the PCR worked. Why

Colony screen them? In all of the cloning I have done, if the screen
is positive, then I am ~95% sure the sequence is fine. There was just
1 or 2 cases where there was an insignificant mutation outside the
region of interest.

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