Re: [DIYbio] Re: qPCR fluorescence detection dynamic range

Can you add the respective run lengths of each protocol?

On Wed, May 22, 2013 at 12:07 PM, Jonathan Cline <jncline@gmail.com> wrote:
>
> On Monday, May 20, 2013 4:05:12 PM UTC-7, Josh W. Perfetto wrote:
>>
>> Ashley, the plan is to provide a very low-cost unit, ....
>>
>> very friendly software accessible
>> via wifi/ethernet/touch screen.
>
>
>
> Low-cost is not synonymous with providing these expensive system components
> (wifi/ethernet/touch screen). Don't even mention using likewise expensive &
> underwhelming arduino. $10 here, $10 there, it all adds up and is not very
> low cost. USB is a better bet, serial still the cheapest and simplest. For
> an automation system, a better bet is industrial grade wired bus with longer
> distance. Lab equipment typically doesn't move or alternatively if it's a
> field unit, it is plugged into a computer after logging data internally.
>
> Here's typical options for low cost communication. It's probably better to
> have independent power rather than run power through the communication link.
>
>
> RS-232 - UART framed, single ended, full duplex, non shared media, NRZ, up
> to a few hundred feet, non powered, non isolated.
>
> RS423 - UART framed, single ended, full duplex, non shared media, logic
> level, several hundred feet, non powered, non isolated.
>
> RS-422 - UART framed, differential, full duplex, non shared media, logic
> level, up to a thousand feet, non powered, non isolated.
>
> RS485 - UART framed, differential, half duplex, shared media, up to a couple
> thousand feet, non powered, non isolated.
>
> CAN/Fieldbus - UART framed, differential, half duplex, NRZ, powered, signal
> can be isolated.
>
> GPIB/IEEE-488 - parallel bus, logic level, short-range, shared media, high
> bandwidth, ruggedized connectors, very common in tech equipment, more
> expensive connectors & cabling in comparison to the others.
>
>
>
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--
-Nathan

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