Re: [DIYbio] Re: qPCR fluorescence detection dynamic range

I think having even a single-tube qPCR machine coupled with some
gelRed or gelGreen would be better than running a gel, because the dye
isn't wasted when you load the sample into a gel well... so you can
see real-time if your reaction is doing ANYTHING AT ALL, then see what
the band sizes are, etc later on the gel.

Sounds like the variability Josiah is talking about is in sample prep,
so just produce the machine at a lower cost and it will still appeal
to the people with the variable protocols that can still pull grant
money.

Josh, simply add a capillary tube in same box with a high-voltage
power supply, and now you've got a reagent-reduced capillary
electropohoresis AND a sensitive optical path in place for detecting
fragment elution time. Now you're setup to increase the capillary
length, and find/formulate cheaper non-agarose gel, and you've got a
sanger sequencer. (Actually I found a paper that acquired 2bp
resolution with agarose, but it wasn't amazing....)

On Thu, May 23, 2013 at 1:50 PM, Josh Perfetto <josh@openpcr.org> wrote:
> On Thu, May 23, 2013 at 11:54 AM, Jonathan Cline <jcline@ieee.org> wrote:
>>
>> Touch screen: how's that work with gloves on? Better make it resistive.
>> Except, no one likes those because they're really annoying to use (pressure,
>> precision), especially now that we're all spoiled by capacitive displays.
>> Worse case you've got a membrane keypad (again, no one likes those) and a
>> non-
>
>
> Doesn't the iPhone/iPad/Android phone work ok for you with gloves on? I
> agree anything less than capacitive is not desirable.
>
>>
>> touch display. Either way you'll have to compromise on display resolution
>> and size, when you really want to graph something onscreen in a really large
>> format. Meanwhile one of the most successful equipment stories is still
>> Nanodrop, which doesn't have a display at all, as I suggested: it sends all
>> data to the nearby computer.
>
>
> Yes, this is why I wanted to have really good IP connectivity so it could be
> controlled from any computer, and data analyzed in a convenient way. There
> were other use cases I wanted to support though, especially someone doing
> the same assay continuously, like in a food testing application. It may be
> easier to select the assay, enter a sample identifier, and hit go in the
> lab, without fussing with a computer.
>>
>>
>> Re: Wireless again. Measuring very low voltages with sensitive
>> electronics while beaming a bunch of RF energy all around right next to the
>> amplifiers will cause trouble.
>
>
> That is a really good point, I am glad you raised it. I had not thought
> about that or tested it specifically. I will try to see what impact it has
> but I think you are right. Perhaps the radio can be depowered when
> measurements are taken.
>
>>
>> Cheaper than competitive equipment? Why not kill off the competing
>> equipment with either a retail price so low that their margins are
>> destroyed, or alternatively keep the "higher low" price as you suggest and
>> keep more margin yourself? Either way the cost of building the device
>> should be lower, not higher.
>
>
> Well that is kindof what I think being an order of magnitude cheaper does,
> except that I am not trying to compete directly with the existing equipment.
> The specs of this device (16 wells, single channel) certainly don't match
> most existing equipment, so it will not be a substitute for many existing
> users. But I think there are many people who currently don't do qPCR, who
> could use this machine despite those limitations, if the price point was
> much lower.
>
> -Josh
>
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--
-Nathan

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