Re: [DIYbio] Re: qPCR fluorescence detection dynamic range

On Thu, May 23, 2013 at 2:00 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
Josh, simply add a capillary tube in same box with a high-voltage
power supply, and now you've got a reagent-reduced capillary
electropohoresis AND a sensitive optical path in place for detecting
fragment elution time. Now you're setup to increase the capillary
length, and find/formulate cheaper non-agarose gel, and you've got a
sanger sequencer. (Actually I found a paper that acquired 2bp
resolution with agarose, but it wasn't amazing....)

 Yes, maybe for version 3 :)

-Josh

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