[DIYbio] Re: Cheap DNA source for PCR control

What type of PCR? If the amplicon is relatively small, say for qPCR, you could also have a synthetic oligonucleotide target made.

On Monday, June 24, 2013 4:43:09 AM UTC-5, Mega wrote:

Hi everyone,

 

a professor of mine told me he'd need Stapphyllococus Aureus and Streptococcus Epidermis DNA to make a PCR as a positive control...

 

Buying from DSMZ would cost some 380€ for 5 ug, so he asked if I had an idea where to get it from cheaply. He would just need 100 ng or so, to be able to run a PCR...

 

 

Obviously, I have no idea, it is biosafety level 2 and I'm not interested in working with potential pathogenic stuff...

He often helped me so now I'd like to help him too...

 

 

Does anyone have an idea where he could get it from?

 

Best,

Andreas

 

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