Re: [DIYbio] MFC isolate culture - ideas for media?

Yep - we made a bunch of Winogradsky columns for the BioCurious Makerfaire booth two years ago. I stuck a wire in the top and one in the bottom, to show that it works as an MFC as well. Think I got something like 42 mV open circuit.

Here's a nice closeup of the Winogradsky column I made:

http://www.meetup.com/BioCurious/photos/3342772/55566962/

You can make one of these in a 2L soda bottle, and stick a syringe through the wall of the bottle to sample the bacteria growing at different spots.

Patrik

On Monday, June 24, 2013 9:04:49 PM UTC-7, Nathan McCorkle wrote:

winogradsky is his name i believe

On Mon, Jun 24, 2013 at 6:08 PM, Dakota Hamill <dko...@gmail.com> wrote:
> Candle jar probably a good idea for a cheap oxygen free environment, though
> I"ve never done one.   A sweet nitrogen flooded make-shift growth chamber
> would be nice to, and nitrogen is really cheap from any gas supply company.
> PDA I use mainly for fungal culture though I have had bacteria grow on it,
> specifically actinomyces.  PDA is usually pH adjusted to 4-5 to inhibit
> bacterial growth so check on that or maybe try a bacterial selective media
> as well.
>
> There is a really cool jar that I forget the name of that is basically just
> a pile of mud from the bottom of a lake you sit in a 2 ft tall glass or
> plastic jar and you can visualize the different bacterial growth types
> (anaerobic vs aerobic, faculative vs obligate etc) and sometimes see color
> based on weather or not they use iron.  I've been googling all possible word
> combinations but I can't find it!!! It's named after a specific dude.
>
>
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-Nathan

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