we wanted to do a test PCR at bosslab, so we found some fungus growing on a plate, ground it up with a pestle in some drain cleaner, and added some master mix and universal barcoding primers. If you are just looking for positive controls, some kind of harmless fungus/microbe +universal primers (very cheap) might be the way to go!
If you want to go with fungi, dakota might be able to comment on the primers we used.On Mon, Jun 24, 2013 at 3:55 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
He's gonna ask the lab keeper for Stapph. Epidermitis, which he may have. I suggested asking him and he said he hasn't done that yet... So your proposal helped already.If anyone knew a cheap way to get also S.Aureus DNA (not the bug, because we only have BSL-1!), that would be nice...
On Mon, Jun 24, 2013 at 4:01 PM, Mega <masterstorm123@gmail.com> wrote:
The synthetic template is a nice idea :)
I guess he would have known if we had a strain at university... but gonna tell him.
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