Well, the lux operon seems to be very difficult to PCR. We tried it several times with brand new phusion (proof-reading) polymerase. No amplification.
Then we tried with phire polymerase, it works after some modification. On Sat, Aug 24, 2013 at 5:28 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
If you're talking about money, it'd probably be cheaper to order it
altogether. Promoters aren't usually that large, although if you want a
*good* one you'd need a bit more length.
On Sat, 24 Aug 2013 08:25:30 -0700 (PDT)
"Mega [Andreas Sturm]" <masterstorm123@gmail.com> wrote:
> Hi everyone,
>
> Does anyone by chance have a plasmid that has some promoter, then
> SalI and then KpnI?
>
> Could trade it against a plasmid or some money or thelike.
>
> Best,
> Andreas
>
>
>
>
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