That sounds feasible too... And they (or similar organisms) are easy to get, when you incubate fruits at room temperature. And easy to cultivate.
Btw., I don't think that an oozyte can "suffer" in the sense of feeling pain. So going in the woods and steeling frog eggs and microscoping them would not cause that much suffering, right?On Sat, Aug 24, 2013 at 10:38 PM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
Provided they are not endangered or protected in your region, then
sure, within the boundaries established to protect vertebrates from
unnecessarily painful research.
Given that frogs remain vertebrates, and can meaningfully suffer, I
sincerely suggest you abandon this line of research, whatever it might
be, and restrict yourself to the old standbys of animal developmental
research; drosophila melanogaster. They are trivial to cultivate, there
are abundant protocols online to handle everything you might want to
achieve, they're cheap, and their capacity to fear or suffer is far
below that of even the simplest vertebrate.
Fruit flies sound bothersome, because they can fly and all, but they
are usually anaesthetised by adding a drop of an alcohol mix to their
cultivation flask prior to opening it.
On Sat, 24 Aug 2013 12:34:20 -0700 (PDT)
> Ok, would be too expensive anyway to get a micromanipulator/
> microinjector device....
>
> But the basic "research" with frog egg cells would be easy to
> accomplish, right?
>
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