Re: [DIYbio] Re: Extract egg cells from "monthly" ? Possible? Ethical?

... And combining a species' nuclear genome with a different species' mitochondrial genome is already considered to be genetic engineering and is certainly illegal in germany and as far as I know in Austria, too. 

Von meinem iPhone gesendet

Am 24.08.2013 um 11:41 schrieb Andreas Sturm <masterstorm123@gmail.com>:

Mhm, seems legit...

http://www.ecocyte-us.com/products_oocytes.html

Here frog egg cells are sold, but I guess you could also collect them from female frogs for free. That does not harm the frog, because she lays them.

So one could at least see egg cells, and experiment a bit. Of course, no genetic engineering involved, as that would be illegal.

On Sat, Aug 24, 2013 at 11:35 AM, Brian Degger <brian.degger@gmail.com> wrote:

It is licenced for a few research groups in uk for cytoplasmic (rabbit/cow egg with human nuceus) hybrids.
Not diy though.

On 24 Aug 2013 10:30, "cat ferguson" <carolinetaylorferguson@gmail.com> wrote:

ASSUMING follicle stimulating drugs can be accessed by laypersons and work on dogs (super unlikely). You'd have to anesthetize the dog, since you have to stick a big needle through its vagina into its follicles, generally guided by an ultrasound, since follicles are tiny. That's not DIY, that's a veterinary procedure. 

Also ew. What. If DIY human-animal SCNT isn't illegal, it probably should be.

—

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On Sat, Aug 24, 2013 at 2:09 AM, Andreas Sturm <masterstorm123@gmail.com> wrote:

Just read through all again, and came to think of something related... Is it feasible to induce over-ovulation in a dog, and then harvest the cells in a diy manner?

Will it hurt the dog? Any side-effects?

And finally, if you had a micromanipulator (quite expensive), could you put human nucleus into dog egg cells to make human stem cells? Mitochondria will be dog mitochondria, but I guess that won't do harm.

Can you buy dog egg cells? Or frog egg cells (ok, inducing human nucleus into a frog egg cell would be yukky)?

On Tue, Aug 13, 2013 at 11:40 PM, Cat Ferguson <carolinetaylorferguson@gmail.com> wrote:

On Tue, Aug 13, 2013 at 1:05 AM, Andreas Sturm <masterstorm123@gmail.com> wrote:

Isn't it that the egg wanders from the ovary into the cervix during ovulation? And by menstruating it is excreted out off the body?

German wikipedia says that each ovulation yields 10-20 follicles (egg cells+accompanying cells). So 9 to 19 egg cells are useless and perhaps be excreted to? Discarded with urine?

...no. Guys. Funny as this has been to read, I think we all need a recap of basic biology.

One egg is released into the oviduct during ovulation, during which it is available briefly for fertilization. During menstruation the uterine lining, which had prepared for a fertilized egg to implant, is shed. The egg degrades, and it's not possible to recover it - and even if it is excreted rather than absorbed, it's no longer going to be fertile. Eggs are only viable 12-24 hours after being released.

IVF makes the ovaries start to develop a ton of extra eggs, which are then harvested by sticking a needle through the vagina and into the follicles (each follicle holds one egg). 

Urine is unrelated to the ovaries, as it comes out of the urethra. I know it's in the same neighborhood, but it doesn't seem that hard to keep them separate. 

--

Cat Ferguson

carolinetaylorferguson@gmail.com

(347) 559-6631

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