Did anyone try out this method? I got the PPY strain from Einstein but don't have luck with it :/
On Friday, September 7, 2012 6:34:17 AM UTC-4, Cathal Garvey wrote:
-- On Friday, September 7, 2012 6:34:17 AM UTC-4, Cathal Garvey wrote:
oooo! I love crude things! :d
Thanks! :)
On 07/09/12 07:36, Nathan McCorkle wrote:
> P.S. this seems like something Cathal would be interested in... since
> it seems like nothing more than a crude cell lysate/extract
>
> On Fri, Sep 7, 2012 at 2:34 AM, Nathan McCorkle <nmz...@gmail.com> wrote:
>> this is a pretty decent method, turns out the folks that did this are
>> right up the street from me... maybe I can talk to them
>>
>> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333860/pdf/ gkr1288.pdf
>>
>> Published online 12 January 2012 Nucleic Acids Research, 2012, Vol.
>> 40, No. 8 e55
>> SLiCE: a novel bacterial cell extract-based DNA cloning method
>> Yongwei Zhang*, Uwe Werling and Winfried Edelmann*
>> Department of Cell Biology, Albert Einstein College of Medicine,
>> Bronx, NY 10461, USA
>> Received July 28, 2011; Revised December 13, 2011; Accepted December 15, 2011
>>
>>
>> Here's to homologous recombination!
>>
>>
>>
>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>
>
>
--
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