In another post that I wrote, "Creating Luminous Plants", I was told of an experiment that takes a pcr machine and uses a chloroplast promoter containing P.Phosphoreum. Here's his exact message :
"What already has been done: Make a PCR of P.Phosphoreum (a glowing marine bacterium) which cut out the Lux Genes (they are very next to each other, so one PCR using 2 primers was enough), add a chloroplast-promoter and insert it into chloroplasts. They are able to express operons, and there we go. It was glowing (weakly, but visible) in fact.
See http://www.plosone.org/
Can I buy/make some of this stuff to try it at home? I notice a few PCR machines on Ebay from time to time for a couple hundred. Where can I buy these photobacterium? Can I experiment with luciferase and other stuff as well? What is a chloroplast-promoter and how can I insert it into the chloroplast of an organism? I'm still interested in possibly doing this project.
Past topic thread.
https://groups.google.com/forum/#!msg/diybio/FpIy91GmyM8/dCjqhMegpxgJ
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