Promoters = Short stretch of DNA that cell machinery(proteins) recognizes and attach to start the transcription (DNA to RNA) of the gene. Genes often have promoters that are unique to them or shared with a couple other genes.
-- It is sort of like an on/off switch. If the switch is gone, the gene won't work. If the switch is there but off, it won't work. If the switch is there and on, the gene works.
On Thursday, October 17, 2013 3:48:59 AM UTC-7, T.J. wrote:
Organisms change the combinations of genes that are turned on and off in a cell depending on its type (ex: heart vs. liver, stem vs. root). By using the chloroplast promoter to control the Lux genes, only cells that have the chloroplast gene activated will glow.
If you are not familiar with the techniques, I would recommend buying a molecular biology lab book on Amazon (used should be around 20). It'll walk you through all of the molecular biology techniques you will need -- from cloning to PCR.
On Thursday, October 17, 2013 3:48:59 AM UTC-7, T.J. wrote:
In another post that I wrote, "Creating Luminous Plants", I was told of an experiment that takes a pcr machine and uses a chloroplast promoter containing P.Phosphoreum. Here's his exact message :
"What already has been done: Make a PCR of P.Phosphoreum (a glowing marine bacterium) which cut out the Lux Genes (they are very next to each other, so one PCR using 2 primers was enough), add a chloroplast-promoter and insert it into chloroplasts. They are able to express operons, and there we go. It was glowing (weakly, but visible) in fact.
See http://www.plosone.org/article/info:doi/10.1371/ "journal.pone.0015461
Can I buy/make some of this stuff to try it at home? I notice a few PCR machines on Ebay from time to time for a couple hundred. Where can I buy these photobacterium? Can I experiment with luciferase and other stuff as well? What is a chloroplast-promoter and how can I insert it into the chloroplast of an organism? I'm still interested in possibly doing this project.
Past topic thread.
https://groups.google.com/forum/#!msg/diybio/ FpIy91GmyM8/dCjqhMegpxgJ
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