Re: [DIYbio] Barcoding of wheat

If you are barcoding the wheat anyway, you could also get a fungal genome isolation kit and try to barcode any fungi present. You would probably have to use next-gen sequencing since you'll have isolated a heterologous mixture of fungal genomes.

uBiome is offering some kind of barcoding service on scienceexchange for $69, but I don't think it's next-gen based. But they might be open to an affordable collaboration. http://widgets.scienceexchange.com/facilities/ubiome/services/16s-rdna-sequencing-analysis

Mac

On Tue, Oct 15, 2013 at 10:55 AM, Simon Quellen Field <sfield@scitoys.com> wrote:

It might be interesting to look at commensal fungi and other organisms that might have aided storage, or selectively degraded some of the grains. There might also be links between commensals and the germination rates of seeds that old (if you get any germination at all).

Measuring the amino acid composition, fatty acid composition, and other nutritional aspects might be interesting. What has been gained over the years, and what has been lost?

Measuring mineral content such as arsenic might tell you about groundwater depletion as a result of agriculture, or changes in local heavy metal concentration as a result of local mining and smelting.

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On Tue, Oct 15, 2013 at 10:19 AM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:

Btw, any other ideas what research to do on 2000 year old grains? :D

we want to put some on soil and grow them :D

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