Re: [DIYbio] DIY nanodrop alternative?

This still only for 600 nm, right? I keep thinking there should be a cheap hack to do a simple 260/280 nm spec for RNA/DNA quantitation and purity testing. Much more demand for that than for a general UV/Vis spectrophotometer. Maybe just a diffraction grating and two UV sensors at fixed angles?

Patrik

On Monday, October 14, 2013 6:39:12 AM UTC-7, Sebastian wrote:
I posted off list by accident. Here was my response to hugo's question:

So far so good. Still working on the integrated microcontroller PCB and
arduino shield alternative. I've been using my prototype for the last
few weeks making competent cells and such and it works quite well. The
only issue is that due to the arduino's crappy clock and edge detection
I lose some edges if you make a reading at the wrong time so I'm not
comfortable charging anything until I get this important issue fixed.
It does not hinder the performance, rather you may have to blank a few
times to get a 100% transmittance reading. I'd like to add a custom
circuit for edge detection so the only thing the arduino needs to do is
output to the LCD and down the USB as serial IO. Will start working on
that issue with more intensity now that I know someone is interested.
There are others on the list working on a similar device so they may
chime in too.


Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Cathal Garvey
Sent: 10/14/2013 9:28 AM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] DIY nanodrop alternative?
Don't go Kickstarter; they've already demonstrated a dislike of
Science. Go with any of the other crowdfunders or selfstarter.

I will be happy to snap one of these up; I still have no working spec
and could use one.

On Mon, 14 Oct 2013 05:37:29 -0700 (PDT)
Hugo Correia <har...@gmail.com> wrote:

> So what's the status on this? Kickstart ready?
>
> On Sunday, 14 July 2013 07:45:36 UTC+2, Sebastian wrote:
> >
> >  I'm in the process of making a very affordable dedicated OD600
> > module soon. I can send you a prototype to demo free of charge. Its
> > all open source. The final kit will be 25 bucks and initial trials
> > are proving quite good. Its fixed at 600nm but will have lots of
> > nice features and the ability to swap modules for other wavelengths
> > or multiple cuvettes. If all goes well I may kick start it to make
> > one for DNA. Ill keep you posted if you'd wish.
> >
> > Sent from my Windows Phone
> >  ------------------------------
> > From: Koeng <javascript:>
> > Sent: 7/14/2013 12:52 AM
> > To: diy...@googlegroups.com <javascript:>
> > Subject: [DIYbio] DIY nanodrop alternative?
> >
> > Hey!
> >
> > I have been looking around for a spectrometer for my lab, and
> > wanted to know if anyone has any recommendations on one. All I need
> > is it to have OD600 and most of all, I want it to be able to sense
> > how much DNA I have in a sample. I am not very experienced with
> > spectrometers, so any suggestions?
> >
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