Re: [DIYbio] Re: Animal slaughter -- keeping it alive in a petri

Patrik, there are some great pics in there!

Do you happen to know if there's a 'before' pic in this arrangement?
http://www.flickr.com/photos/yeahsnos/10006833654/sizes/o/in/pool-2303889@N24/

What were you going for with the proteases? Wouldn't that destroy the
ECM, the scaffold?

I found some PBS (phosphate buffered saline) on amazon.com for
~$5/liter (when bought in 10L quantities)... but I think I'll just
make up some sterile isotonic salt solution with NaCl and KCl from the
food store (for rinsing the heart chunk of blood). Not sure how long
the cells would survive just in saline, though I haven't looked into
easy/DIY growth media. I figure I could spin down some blood and
collect the plasma, but I think I'd still need to filter that to get
'serum'.

Maybe it will be enough to keep the cells happy for a day or two... I
seem to remember my chicken explant and trypsinized culture started
beating within a day or two.

On Fri, Oct 11, 2013 at 1:18 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
> Hi Nathan!
>
> Just did some experiments in decellularization two weeks ago. We were going
> to do a whole pig heart, but the heart we'd gotten was missing to much of
> the vasculature to do the perfusion.
>
> We did get to try some health food store digestive enzymes on heart slices -
> both bromelain and papain worked pretty darn well. We didn't try to grow any
> cells on them afterwards, of course...
>
> Here's some pics - still need to add a bunch more:
>
> http://www.flickr.com/groups/2303889@N24/pool
>
> Patrik
>
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--
-Nathan

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