On Saturday, October 19, 2013 7:48:53 AM UTC-7, eleitl wrote:
On Fri, Oct 11, 2013 at 01:18:34PM -0700, Patrik D'haeseleer wrote:
> Hi Nathan!
>
> Just did some experiments in decellularization two weeks ago. We were going
> to do a whole pig heart, but the heart we'd gotten was missing to much of
> the vasculature to do the perfusion.
Hey, I missed that one the first time, for some reason.
What is the procedure by which you obtained your heart? Is it legal
to obtain animal organs from slaugherhouse for research purposes
without a permit in your jurisdiction? How long are the ischemic times
in your experience, and how badly are the organs mangled to the
point where they're useless?
I got the original hearts via Cameron, one of our BioCurious members whose sister has a farm where they raise pigs. Unfortunately, the heart was butchered to be used as food, which means they cut off the major arteries, and put a slit through the chambers to let the blood drain. Sigh...
I'm getting five (5!) fresh pig hearts tomorrow, from a meat wholesaler in Berkeley that was recommended bythe good folks at the California Academy of Sciences. Apparently, the trick is to ask for a "bio-heart", meaning a heart that is intended for scientific experiments. The tradeoff is that they are not certified as food-grade - presumably because USDA regulations specify that you have to cut off the inedible bits.
Patrik
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