Re: [DIYbio] Re: PCR 96-well plate mats?

so do people use it for regular PCR or just qPCR? I have a heated lid
so I can't understand why someone would use it in a regular PCR block.

On Wed, Oct 9, 2013 at 9:58 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> This explains a bit:
> http://www.4ti.co.uk/pcr-consumables-and-accessories/pcr-accessories/optical-film-compression-pad/
>
> basically it's a foam pad in case your multiwell tray isn't perfectly
> flat, in case the clear adhesive film (which seals in moisture) starts
> bubbling if you mess up when applying it.
>
> On Wed, Oct 9, 2013 at 4:11 PM, Jeswin <phillyj101@gmail.com> wrote:
>> Actually, it looks like this and I think that's what she had:
>>
>> http://www.bio-rad.com/en-us/sku/adr-3296-optical-compression-pad
>>
>> Optical Compression Pad which is for qPCRs and I have no idea why you
>> would use that in a reguar PCR machine.
>>
>>
>> On Wed, Oct 9, 2013 at 7:03 PM, Jeswin <phillyj101@gmail.com> wrote:
>>> A colleague wanted to use my PCR machine for a 96 well plate but did
>>> not because she couldn't find a "mat" to place on top of the plate.
>>>
>>> I never used a 96 well plate in the cycler before so I was a bit
>>> confused about what she was talking about. It's not a sealing mat with
>>> indentations. It's a flexible, "rubbery", "mat" with holes (96, I
>>> guess) that's a few millimeter thick. Anyone know what it is?
>>>
>>> She said it was for keeping to all tight but I don't think it was
>>> necessary. The machine I use has a lid with different size settings
>>> (round top tubes, flat, well plate).
>>>
>>> So you guys use that thing and is it really needed?
>>>
>>> Thanks
>>
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>
>
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