Re: [DIYbio] Re: SLICE -- Seamless Ligation Cloning Extract... or, using lambda phage homologous recombination in-vitro

I too got this strain and no luck, but I'm no pro.  Anyone else have trouble or success?  A recent article on synthetic assembly methods stated that of survey respondents, around 10%, or 13 respondents, used SLICE.  I might try CPEC instead, and I really want ABI-REC to work, but so far no luck...

On Wednesday, September 18, 2013 4:08:05 PM UTC-7, Nathan McCorkle wrote:
On Thu, Sep 12, 2013 at 2:54 PM, yamwa <yam...@gmail.com> wrote:
> Did anyone try out this method? I got the PPY strain from Einstein but don't
> have luck with it :/

Didn't have luck in what sense? How long were your homologous flanking regions?

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