Hi!
While waiting for my construct / primers (pGlowroplast + yeast fluorescent protein) I was thinking about doing something in the meantime…
http://www.pnas.org/content/102/44/15971.full.pdf
This paper describes how CyanoBacillus was made…
Now I was thinking if I can fuse Cyanobacteria and Bacillus Subtilis. But through natural breeding (in a legal sense). I heard Cyanos are resistant to ampicillin naturally. I read Bacillus subtilis sometimes have a natural resistance against rifampicin (but where to get such a strain?).
Now incubate both bacteria in PEG (do I need to make protoplasts?) to make them fuse. Plate on Amp+Rif LB so the cells are forced to keep two chromosomes. Maybe the chromosomes will fuse at some point? After some time?
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