Re: [DIYbio] Small side project - baking GFP bread - exhibtion

I support any pklac2-based projects!

On Sun, Nov 10, 2013 at 2:29 AM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> Hi everyone,
>
> I am condsidering as a "weekend project" to add GFP to a yeast
> (saccharomyces, considered GRAS as you know), and then bake bread with it.
> Haven't talked to the guys from Ars Electronica yet if their Biolab would
> host this (but I'm positive, if it is legal they will allow it).
>
> Therefore I would subclone GFP into pKlac2 (
> https://www.neb.com/~/media/Catalog/All-Products/6F454BB8401043D798342A0719273945/Datacards%20or%20Manuals/N3742Datasheet-Lot0011210.pdf
> ) fused to the alpha-mating factor which is then cleaved off.
>
> The GFP would then be secreted into the medium (bread dough). So if you cut
> the dough, there will be small holes which will be fluorescent.
>
> What is questiuonable, will the GFP still work? If it is secreted the yeast
> will glycosylate it, unlike most cytosolic proteins (according to
> http://wolfson.huji.ac.il/purification/PDF/Expression_Systems/NEB_Klactiskit_manual.pdf
> )
> Should I express it in the cytosol - no golgi etc.?
>
>
> Thanks,
> Andreas
>
> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+unsubscribe@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/3472ed72-4d98-4229-9b89-5fa7b6163b09%40googlegroups.com.
> For more options, visit https://groups.google.com/groups/opt_out.



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9L3eX2hrFQCVbt2BNDyNG%3DZgFX5VHrS4cy%2BrioMcgcsQA%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.