Well, they thought a commercial typhoon laser scanner was like $100k+, so $1-2k BOM made sense to them. Just depends on how DIY you want to get.
On Friday, December 6, 2013, Brian Degger wrote:
the costs of the optics was still fairly high, think $600-$1000 (havent done the adding up)from the bom on http://2013.igem.org/Team:TU-Delft/Zephyr_How
520nm Bandpass Filter, 36nm Bandpass, OD6 Blocking, 25mm Dia, Stock No. #67-030 Emission filter 1 link 1 472nm Bandpass Filter, 30nm Bandpass, OD6 Blocking, 25mm Dia, Stock No. #67-027 Excitation filter 1 link 2 495nm Dichroic Filter, 25.2 x 35.6mm, Stock No. #67-079 Dichroic mirror 1 link 3 4X DIN Plan Commercial Grade Objective, Stock No. #67-706 Objective 1 link 4 10X DIN Wide Field Microscope Eyepiece, Stock No. #36-130 Eyepiece 1 link 5 --On Fri, Dec 6, 2013 at 4:19 PM, Mac Cowell <mac@diybio.org> wrote:
You could start with this affordable 2000x microscope: http://www.amazon.com/gp/aw/d/B004QEFO1Q
The TU DELFT iGEM 2013 team prototyped a diy "typhoon" fluorescence scanning microscope they called zephyr. The XY stage was superfluous but I thought the optics they figured out was really interesting. The got a well-matched high-power LED and dicroic mirror and emission filter and mounted them in a simple integrated light path. Perhaps those components could be combined with the camera mount of the above microscope.
http://2013.igem.org/Team:TU-Delft/Zephyr
Mac
On Friday, December 6, 2013, Andreas Stuermer wrote:To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2BiLdW2F%3D2pbrY1Bw9ZpOxQLiQn2TyXZmXNJ%3Dx8H3zb8cxmiOA%40mail.gmail.com.> I wonder if visible UV can damage eyes...the 390nm spectral ballpark range.Probably yes, but not as much as higher energy UV. Wear sunglases when looking into the microscope?On Fri, Dec 6, 2013 at 2:00 PM, Sebastian Cocioba <scocioba@gmail.com> wrote:
Electron Microscopy Sciences sells an adapter for dissection microscopes to become UV. Its just a flex-light with UV LED. I'm sure you could easily hack some things together to make a similar product. Have you thought of using the 3d printable microscope and add a blue LED like previously suggested? I wonder if visible UV can damage eyes...the 390nm spectral ballpark range.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Andreas Stuermer
Sent: 12/6/2013 7:10 AM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: $240 fluorescence microscopeNice idea ;)Will get my home gel illuminator soon anyway.On Fri, Dec 6, 2013 at 11:57 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
Take blue LED gel illuminator and place it under microscope with
sample in place of a gel?
On Fri, Dec 6, 2013 at 2:46 AM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> Reviving this old thread :D
>
> Does anyone have an idea where to get a cheap flurescence microscope? The
> "cheapest" one on ebay is ~900$.
>
> I could hack my own cheap microscope but a) possibility to destroy it, b)
> possibility to burn my eyes with the uv light...
>
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