Yeah, the same is true also for HOK/SOK.
But problem is the proteins are not excreted into the medium. Thus it does not kill other bacteria that haven't taken up the plasmid. And that should be the goal :)
If you fuse it to a signal pepptide thatdirects it outside the cell, the problem is
a) other bacteria won't take it up easily (and it ususally inhibits mRNA translation)
b) if another bacteria would take it up, it would recognize the signal peptide and excrete it.
Antibiotics usually are more or less small carbon scaffolds, which can freely diffuse through the membrane.
-- But problem is the proteins are not excreted into the medium. Thus it does not kill other bacteria that haven't taken up the plasmid. And that should be the goal :)
If you fuse it to a signal pepptide thatdirects it outside the cell, the problem is
a) other bacteria won't take it up easily (and it ususally inhibits mRNA translation)
b) if another bacteria would take it up, it would recognize the signal peptide and excrete it.
Antibiotics usually are more or less small carbon scaffolds, which can freely diffuse through the membrane.
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