Re: [DIYbio] Bacterocins as an alternative selection method

OK cool.

I got it now. You know I can help with shipping in US. I have legal biz entity and FedEx account. Can tranship or drop ship if that will help anyone. If it took off I could also bulk buy for you guys too.

>matt

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Koeng <koen...@gmail.com> wrote:

Just being able to produce an antibiotic in a DIY situation can definitely help with learning about biology and yes, off the grid would be nice because these companies really hate shipping to houses

On Sunday, January 12, 2014 3:12:02 PM UTC-8, incisive systematics wrote:

OK....

Still lost on why. Is kanamycin such an issue, or is DIY about being totally off the grid? I get KanR but synthesis ......

>matt

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Koeng <koen...@gmail.com> wrote:

Synthesize kanamycin synthesis and resistance genes and put them on a plasmids, therefore the plasmid selects for itself

On Sunday, January 12, 2014 2:56:52 PM UTC-8, incisive systematics wrote:

OK I'm confused. Synthesizing kanamycin? Really? Why?

Or kanamycin resistance genes?

>matt

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Keoni Gandall <koen...@gmail.com> wrote:

I'll wait till then then!
On Jan 12, 2014, at 10:31 AM, Cathal Garvey <cathal...@cathalgarvey.me> wrote:

> :) Give me a week, and I'll have a fun project to share with you!
>
> On 12/01/14 18:24, Koeng wrote:
>> Hello everyone!
>>
>> Recently there was talk of synthesizing Kanamycin production operon for use
>> in diy biology. I wanted to know if anyone had looked into E coli
>> bacterocins as an alternative. They would be cheaper to synthesize, and
>> even better the strain to get them from could possibly come from a genetic
>> stock center. Probably the best part is they are almost guaranteed  to work
>> in E coli. Just an interesting research option
>>
>> -Koeng
>>
> <0x988B9099.asc>

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