Re: [DIYbio] GFP visibility vs concentration?

you might try finding the dosage (watts of light per area... i.e.
w/cm^2) that photobleaching of GFP occurs at, then from there
determine the maximum fluorescence (watts of light per area, or # of
photons), then find the minimum detection limit for the eye for that
wavelength (watts/area or photons).

With that you should be able to determine how much light a single GFP
gives off at full brightness, then you can divide the minimum
sensitivity of the eye by that number to get # of GFPs needed. You
could then modify the equations with lower input power, in case you
don't have a super-bright UV light that is capable of photo-bleaching.

On Thu, Jan 16, 2014 at 2:26 PM, kenny kostenbader
<kensteroo22@gmail.com> wrote:
> Hi All,
>
> I was wondering how much GFP (in aqueous solution) would be required to glow
> bright enough to see with the naked eye? Or does it depend more on the
> intensity/color of the excitation lighting?
>
>
> Thanks!
> Kenny
>
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-Nathan

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