Re: [DIYbio] GFP visibility vs concentration?

Based on this:
http://www.ecse.rpi.edu/~schubert/Light-Emitting-Diodes-dot-org/Sample-Chapter.pdf

and the data on quantum yield
(http://en.wikipedia.org/wiki/Fluorescence#Quantum_yield) here
http://en.wikipedia.org/wiki/Green_fluorescent_protein#GFP_derivatives

you should be able to determine what you want, or be very close to
having all the pieces to get what you want.

On Thu, Jan 16, 2014 at 7:35 PM, kenny kostenbader
<kensteroo22@gmail.com> wrote:
> Nathan, remind me to come back to you when I have a scientific heavy-lifting
> problem on my hands :) that's quite the solution!
>
> Avery, I'd be able to get a value for mg/ml of protein from an absorbance
> reading.
>
> I was hoping to find a set of pictures that compare jars of GFP with
> differing concentrations, something like that. When I get to my own samples,
> I'd be happy to perform some dilutions and post pictures somewhere. Someone
> out there will find that interesting :)
>
>
> Kenny
>
>
> On Thursday, January 16, 2014 7:50:08 PM UTC-5, Avery wrote:
>>
>> or you could get some GFP and serially dilute it until you cant see it.
>>
>> Filters help block the light that causes the excitement, but also make the
>> overall image dimmer.
>>
>> How do you measure GFPs/volume?
>>
>> --A
>>
>>
>> On Thu, Jan 16, 2014 at 6:17 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
>>>
>>> you might try finding the dosage (watts of light per area... i.e.
>>> w/cm^2) that photobleaching of GFP occurs at, then from there
>>> determine the maximum fluorescence (watts of light per area, or # of
>>> photons), then find the minimum detection limit for the eye for that
>>> wavelength (watts/area or photons).
>>>
>>> With that you should be able to determine how much light a single GFP
>>> gives off at full brightness, then you can divide the minimum
>>> sensitivity of the eye by that number to get # of GFPs needed. You
>>> could then modify the equations with lower input power, in case you
>>> don't have a super-bright UV light that is capable of photo-bleaching.
>>>
>>> On Thu, Jan 16, 2014 at 2:26 PM, kenny kostenbader
>>> <kenst...@gmail.com> wrote:
>>> > Hi All,
>>> >
>>> > I was wondering how much GFP (in aqueous solution) would be required to
>>> > glow
>>> > bright enough to see with the naked eye? Or does it depend more on the
>>> > intensity/color of the excitation lighting?
>>> >
>>> >
>>> > Thanks!
>>> > Kenny
>>> >
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>>
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--
-Nathan

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