Nathan, remind me to come back to you when I have a scientific heavy-lifting problem on my hands :) that's quite the solution!
On Thursday, January 16, 2014 7:50:08 PM UTC-5, Avery wrote:
-- Avery, I'd be able to get a value for mg/ml of protein from an absorbance reading.
I was hoping to find a set of pictures that compare jars of GFP with differing concentrations, something like that. When I get to my own samples, I'd be happy to perform some dilutions and post pictures somewhere. Someone out there will find that interesting :)
Kenny
On Thursday, January 16, 2014 7:50:08 PM UTC-5, Avery wrote:
--AHow do you measure GFPs/volume?or you could get some GFP and serially dilute it until you cant see it.Filters help block the light that causes the excitement, but also make the overall image dimmer.On Thu, Jan 16, 2014 at 6:17 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
you might try finding the dosage (watts of light per area... i.e.
w/cm^2) that photobleaching of GFP occurs at, then from there
determine the maximum fluorescence (watts of light per area, or # of
photons), then find the minimum detection limit for the eye for that
wavelength (watts/area or photons).
With that you should be able to determine how much light a single GFP
gives off at full brightness, then you can divide the minimum
sensitivity of the eye by that number to get # of GFPs needed. You
could then modify the equations with lower input power, in case you
don't have a super-bright UV light that is capable of photo-bleaching.
--
On Thu, Jan 16, 2014 at 2:26 PM, kenny kostenbader
<kenst...@gmail.com> wrote:
> Hi All,
>
> I was wondering how much GFP (in aqueous solution) would be required to glow
> bright enough to see with the naked eye? Or does it depend more on the
> intensity/color of the excitation lighting?
>
>
> Thanks!
> Kenny
>
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