Good point. I'm so used to bringing everything in the chemistry lab up in DMSO for LCMS analysis (since it dissolves everything) that I didn't think of intrinsic toxicity of the DMSO at that high of a concentration. Generally we have samples in MeOH which we then let evaporate before plating the dry discs. I spoke to a postdoc at a lab in yale and they say they sometimes just plate straight acetonitrile/water mobile phase extracts on the agar and let it soak in before plating a lawn on top of it. Nevertheless, a 20uL DMSO only control should have been plated. Back to the lab this weekend to re-test! Thanks for the good catch
On Thu, Feb 6, 2014 at 7:21 AM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
Really cool! How do you separate the toxicity of the DMSO itself from
the extract, though? I thought the discs were usually dry before placing
on agar?
--
On 06/02/14 02:57, Dakota Hamill wrote:
> http://basementbiotech.org/successful-disc-diffusion-assay-with-an-endophyte-fermentation-extract/
>
> Thought some people might find it interesting. It aint molecular biology
> or ground breaking science, but it's been pretty cool to use nothing but
> really basic techniques and ghetto equipment to prove that yes, it is
> possible to isolate bio-active secondary metabolites from organisms you
> find in the wild.
>
> Keep on doing science!
>
> -Dakota
>
Please help support my crowdfunding campaign, IndieBB: Currently at
19.6% of funding goal, with 36 days left:
http://igg.me/at/yourfirstgmo/x/4252296
T: @onetruecathal, @IndieBBDNA
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