Re: [DIYbio] Ultra-Cheap DNA Printing/Sequencing

With raman detection and sufficiently dilute dNTPS, you could
potentially use terminal transferase. Sourcing the dNTPs would be akin
to the recent thread on purifying the triphosphates... I remember you
comparing that to cleaning up agar into agarose at home, and how it
wouldn't really be efficient!

On Fri, Feb 21, 2014 at 5:10 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Hm, I happen to know a Raman expert! :)
> For me though, while better chemical methods and nanotechnology stuff is
> great, I'm a sucker for "self-bootstrapping systems". That's part of
> what makes biology awesome, after all.
>
> So, I won't be happy until we have a biological DNA synthesis reaction
> or system that can be home-brewed in its entirity! Everything until then
> is a stopgap!
>
> On 22/02/14 00:54, Nathan McCorkle wrote:
>> you don't need some new kind of laser to do that though, you just need
>> the right optical know-how and a decent detector setup. Read the
>> patents by Helicos and you'll find plenty of similar setups in
>> academic literature:
>> https://groups.google.com/forum/#!topic/diybio/Ke7BqPUD46A
>>
>> You can label-free detection by moving to a Raman setup.
>>
>> On Fri, Feb 21, 2014 at 4:37 PM, Cathal Garvey
>> <cathalgarvey@cathalgarvey.me> wrote:
>>> Well, Sung (@bookhling) can do nano-lasers. I recall he did bio-nanodots
>>> and got them to lase. Crazyballs.
>>>
>>> On 22/02/14 00:32, John Griessen wrote:
>>>> On 02/21/2014 01:40 PM, L wrote:
>>>>> The resulting oglios would then be preferentially selected by
>>>>> sequencing all and destroying the ones that don't match the desired
>>>>> sequence.
>>>>>
>>>>> So basically, yes.
>>>>
>>>> But, nanofabbing of nano-lasers, or even lenses that can aim at a nano
>>>> speck of anything?
>>>> Not in my bag of tricks...
>>>>
>>>> Cathal Garvey wrote:
>>>>> An alternative reaction without chain-branching, which can be recovered
>>>>> as pure DNA using PCR, would be great.
>>>>
>>>> But, following a published method like,
>>>>
>>>> "I've seen at least one paper on this but lost it, and forgot the
>>>> keywords! - It used a DNA analogue
>>>> for the synthesis step, which was compatible enough with DNA Polymerases
>>>> that it could be PCR'd into real DNA afterwards."
>>>>
>>>> seems less imaginary. Thermo-cyclers we can do. Nano-lasers NOT (yet...)
>>>>
>>>
>>> --
>>> Please help support my crowdfunding campaign, IndieBB: Currently at
>>> 26.2% of funding goal, with 20 days left:
>>> http://igg.me/at/yourfirstgmo/x/4252296
>>> T: @onetruecathal, @IndieBBDNA
>>> P: +3538763663185
>>> W: http://indiebiotech.com
>>
>>
>>
>
> --
> Please help support my crowdfunding campaign, IndieBB: Currently at
> 26.4% of funding goal, with 20 days left:
> http://igg.me/at/yourfirstgmo/x/4252296
> T: @onetruecathal, @IndieBBDNA
> P: +3538763663185
> W: http://indiebiotech.com



--
-Nathan

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9L8R0-6%3Dk3TjNVL81_BmtOpjdRULrnyne13oq6ufnUiHg%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.