Maybe this island is the remnant of its ancestors. That then duplicated and mutated.
-- If you have the minimal genome synthesized at one time, remember that you'll need a marker for the cells. I recommend lux :D
So the minimal genome bacillus would glow by default (already very cool! but, it's getting even better - wait for it). When you do homologous integration, by knocking in your gene of interest you would knock out luxAB. So you can see that your experiment worked. :D
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